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primary antibodies for connexin40 (cx40)  (Alpha Diagnostics)

 
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    Structured Review

    Alpha Diagnostics primary antibodies for connexin40 (cx40)
    Primary Antibodies For Connexin40 (Cx40), supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+connexin40+(cx40/anti+connexin40/pm35151687-192-15-20
    Average 90 stars, based on 1 article reviews
    primary antibodies for connexin40 (cx40) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia.
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Expressing:

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia.
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Real-time Polymerase Chain Reaction:

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia.
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Quantitation Assay:

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia.
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Staining:

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia.
    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).



    Similar Products

    primary antibodies for connexin40 (cx40)  (Alpha Diagnostics)
    90
    Alpha Diagnostics primary antibodies for connexin40 (cx40)
    Primary Antibodies For Connexin40 (Cx40), supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+connexin40+(cx40/anti+connexin40/pm35151687-192-15-20
    Average 90 stars, based on 1 article reviews
    primary antibodies for connexin40 (cx40) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    primary antibodies connexin40 (cx40  (Alpha Diagnostics)
    90
    Alpha Diagnostics primary antibodies connexin40 (cx40
    Reduced expression of cardiac conduction system markers in Chkb -deficient mice. RT–quantitative PCR analysis was used to monitor gene expression of atrial natriuretic peptide ( ANP ) ( A ), natriuretic peptide receptor-A ( NPRA ) ( B ), ventricular conduction system markers <t>connexin</t> <t>40</t> ( <t>Cx40</t> ) ( C ), and hyperpolarization-activated cyclic nucleotide-gated channel-4 ( HCN4 ) ( D ). Expression levels were normalized to Gapdh via the ΔΔC T method. n = 3 to 6 mice per group, each bar represents mean ± SD, ∗ p < 0.01, ∗∗ p < 0.01; one-way ANOVA with Tukey’s multiple comparisons post hoc test. E and F , representative images and quantitation of cardiac muscle sections of 30-day-old Chkb +/+ , Chkb +/ − , and Chkb − / − mice stained with sarcomeric myosin MF20 ( green ) and Cx40 antibodies ( red ) along with a Bodipy nuclear stain. The scale bar represents 50 μM.
    Primary Antibodies Connexin40 (Cx40, supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+connexin40+(cx40/anti+connexin40/pmc08913350-262-15-20
    Average 90 stars, based on 1 article reviews
    primary antibodies connexin40 (cx40 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Reduced expression of cardiac conduction system markers in Chkb -deficient mice. RT–quantitative PCR analysis was used to monitor gene expression of atrial natriuretic peptide ( ANP ) ( A ), natriuretic peptide receptor-A ( NPRA ) ( B ), ventricular conduction system markers connexin 40 ( Cx40 ) ( C ), and hyperpolarization-activated cyclic nucleotide-gated channel-4 ( HCN4 ) ( D ). Expression levels were normalized to Gapdh via the ΔΔC T method. n = 3 to 6 mice per group, each bar represents mean ± SD, ∗ p < 0.01, ∗∗ p < 0.01; one-way ANOVA with Tukey’s multiple comparisons post hoc test. E and F , representative images and quantitation of cardiac muscle sections of 30-day-old Chkb +/+ , Chkb +/ − , and Chkb − / − mice stained with sarcomeric myosin MF20 ( green ) and Cx40 antibodies ( red ) along with a Bodipy nuclear stain. The scale bar represents 50 μM.

    Journal: The Journal of Biological Chemistry

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia

    doi: 10.1016/j.jbc.2022.101716

    Figure Lengend Snippet: Reduced expression of cardiac conduction system markers in Chkb -deficient mice. RT–quantitative PCR analysis was used to monitor gene expression of atrial natriuretic peptide ( ANP ) ( A ), natriuretic peptide receptor-A ( NPRA ) ( B ), ventricular conduction system markers connexin 40 ( Cx40 ) ( C ), and hyperpolarization-activated cyclic nucleotide-gated channel-4 ( HCN4 ) ( D ). Expression levels were normalized to Gapdh via the ΔΔC T method. n = 3 to 6 mice per group, each bar represents mean ± SD, ∗ p < 0.01, ∗∗ p < 0.01; one-way ANOVA with Tukey’s multiple comparisons post hoc test. E and F , representative images and quantitation of cardiac muscle sections of 30-day-old Chkb +/+ , Chkb +/ − , and Chkb − / − mice stained with sarcomeric myosin MF20 ( green ) and Cx40 antibodies ( red ) along with a Bodipy nuclear stain. The scale bar represents 50 μM.

    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat# IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitation Assay, Staining

    Summary of cardiac events and their potential drivers due to Chkb deficiency. This study is the first to report that both heterozygous and homozygous Chkb (Choline kinase beta) deficiencies alter the cardiac lipid profile and membrane composition and are associated with cardiomyopathy. Chkb deficiency results in a significant decrease in the expression of ANP , its receptor NPRA , as well as ventricular conduction system markers (hyperpolarization-activated cyclic nucleotide-gated channel-4 [ HCN4 ] and connexin 40 [ Cx40 ]) in Chkb +/− and Chkb −/− mice. ANP expression has been shown to protect against the development of heart failure and is involved in the development of the embryonic ventricular conduction system. Defects in cardiac conduction system development in patients with congenital heart diseases can cause arrhythmias and may lead to sudden death. The decreased capacity of cardiac mitochondria from Chkb −/− mice to utilize fatty acids for oxygen production results in accumulation of AcCa in cardiac muscle. Increased levels of long-chain AcCa have been associated with cardiovascular disease risk, heart failure, left ventricle remodeling and function proportional to disease stage and severity. Furthermore, the alterations in specific cardiac signaling pathways in Chkb -deficient hearts (decreased p-AKT, p-GSKβ, and p-AMPK) lead to defective response to extracellular stimuli and render the hearts more susceptible to cardiomyopathy.

    Journal: The Journal of Biological Chemistry

    Article Title: A mouse model of inherited choline kinase β-deficiency presents with specific cardiac abnormalities and a predisposition to arrhythmia

    doi: 10.1016/j.jbc.2022.101716

    Figure Lengend Snippet: Summary of cardiac events and their potential drivers due to Chkb deficiency. This study is the first to report that both heterozygous and homozygous Chkb (Choline kinase beta) deficiencies alter the cardiac lipid profile and membrane composition and are associated with cardiomyopathy. Chkb deficiency results in a significant decrease in the expression of ANP , its receptor NPRA , as well as ventricular conduction system markers (hyperpolarization-activated cyclic nucleotide-gated channel-4 [ HCN4 ] and connexin 40 [ Cx40 ]) in Chkb +/− and Chkb −/− mice. ANP expression has been shown to protect against the development of heart failure and is involved in the development of the embryonic ventricular conduction system. Defects in cardiac conduction system development in patients with congenital heart diseases can cause arrhythmias and may lead to sudden death. The decreased capacity of cardiac mitochondria from Chkb −/− mice to utilize fatty acids for oxygen production results in accumulation of AcCa in cardiac muscle. Increased levels of long-chain AcCa have been associated with cardiovascular disease risk, heart failure, left ventricle remodeling and function proportional to disease stage and severity. Furthermore, the alterations in specific cardiac signaling pathways in Chkb -deficient hearts (decreased p-AKT, p-GSKβ, and p-AMPK) lead to defective response to extracellular stimuli and render the hearts more susceptible to cardiomyopathy.

    Article Snippet: After 1 h, blocking buffer solution was removed and replaced with blocking buffer containing primary antibodies for Connexin40 (Cx40) (1:50, Alpha Diagnostics, Cat# IGG1 CX40A), Sarcomeric myosin (MF20) (1:50, Developmental Studies Hybridoma Bank, Cat# MF-20).

    Techniques: Expressing